Protection from Fas-Mediated Apoptosis by a Soluble Form of the Fas Molecule

Jianhua Cheng; Tong Zhou; Changdan Liu; John P. Shapiro; Matthew J. Brauer; Michael Kiefer; Philip J. Barr; John D. Mountz

doi:10.1126/science.7510905

Abstract

Fas is an apoptosis-signaling receptor molecule on the surface of a number of cell types. Molecular cloning and nucleotide sequence analysis revealed a human Fas messenger RNA variant capable of encoding a soluble Fas molecule lacking the transmembrane domain because of the deletion of an exon encoding this region. The expression of soluble Fas was confirmed by flow cytometry and immunocytochemical analysis. Supernatants from cells transfected with the variant messenger RNA blocked apoptosis induced by the antibody to Fas. Levels of soluble Fas were elevated in patients with systemic lupus erythematosus, and mice injected with soluble Fas displayed autoimmune features.

Keywords

Fas ligandApoptosisFas receptorMolecular biologyMessenger RNAFlow cytometryTransfectionExonChemistryDeath domainReceptorBiologyAntibodyComplementary DNAGeneCell biologyProgrammed cell deathImmunologyBiochemistry

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Publication Info

Year: 1994
Type: article
Volume: 263
Issue: 5154
Pages: 1759-1762
Citations: 1119
Access: Closed

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APA Style

                            
                                
                                    Jianhua Cheng, 
                                
                                    Tong Zhou, 
                                
                                    Changdan Liu
                                
                                et al.
                            
                            (1994). 
                            Protection from Fas-Mediated Apoptosis by a Soluble Form of the Fas Molecule. 
                            Science
                            , 263
                            (5154)
                            , 1759-1762.
                            https://doi.org/10.1126/science.7510905
                        

Identifiers

DOI: 10.1126/science.7510905