Abstract
Potassium permanganate reacts selectively with thymidine residues in DNA (1) while hydroxylamine hydrochloride at pH 6 specifically attacks cytosine (2). We have adopted these reactions for use with the chemical sequencing method developed by Maxam and Gilbert (3).
Keywords
BiologyCytosineDNAHydroxylamine HydrochloridePotassium permanganateDNA sequencingThymidineHydroxylaminePyrimidineBiochemistryNucleic acidOrganic chemistryChemistry
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Publication Info
- Year
- 1980
- Type
- article
- Volume
- 8
- Issue
- 20
- Pages
- 4613-4620
- Citations
- 318
- Access
- Closed
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Cite This
Carol M. Rubin,
Carl W. Schmid
(1980).
Pyrimidine-specific chemical reactions useful for DNA sequencing.
Nucleic Acids Research
, 8
(20)
, 4613-4620.
https://doi.org/10.1093/nar/8.20.4613
Identifiers
- DOI
- 10.1093/nar/8.20.4613