Abstract
A method is described for the identification and determination of sphingolipid long-chain bases in various animal and plant lipids. Sphingosine and related bases, isolated as a mixture from acid hydrolysates of sphingolipids, are oxidized by sodium metaperiodate and the fatty aldehyde reaction products are isolated and analyzed by gas-liquid partition chromatography. The records thus obtained reflect the composition of the sphingolipid base fraction. A preliminary survey of various tissues is reported and the types of long-chain base found for each tissue are given. Evidence is presented for the presence of a new long-chain base associatcd with the sphingomyelin fraction of human plasma lipids.
Keywords
MicroanalysisSphingolipidComputational biologyChemistryComputer scienceBiologyBiochemistry
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Publication Info
- Year
- 1959
- Type
- article
- Volume
- 1
- Issue
- 1
- Pages
- 40-47
- Citations
- 350
- Access
- Closed
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Cite This
Charles C. Sweeley,
Ezio A. Moscatelli
(1959).
Qualitative microanalysis and estimation of sphingolipid bases.
Journal of Lipid Research
, 1
(1)
, 40-47.
https://doi.org/10.1016/s0022-2275(20)39090-8
Identifiers
- DOI
- 10.1016/s0022-2275(20)39090-8