Abstract
Annexin I is an abundant cytosolic protein in human neutrophils. Besides its intracellular location, annexin I is found as an extracellular protein and the pathway for secretion has been of interest since the protein lacks a signal sequence for secretion. It was recently shown that annexin I is stored in the secretory gelatinase granules of human neutrophils, suggesting that the protein might be released through a granule mobilisation and fusion process resembling classical secretion. In this study we have determined the intracellular localisation of annexin I in human neutrophils using subcellular fractionation, protein separation by SDS‐PAGE and immunoblotting, and show that virtually all annexin I is localised in the cell cytosol.
Keywords
CytosolSecretionIntracellularExtracellularAnnexin A2Cell biologyAnnexinCell fractionationSubcellular localizationGelatinaseSignal peptideGranule (geology)Secretory proteinBiologyAnnexin A1BiochemistryCytoplasmCellPeptide sequenceEnzymeGene
Affiliated Institutions
Related Publications
Novel features in the genetic code and codon reading patterns in <i>Neurospora crassa</i> mitochondria based on sequences of six mitochondrial tRNAs
We report the sequences of Neurospora crassa mitochondrial alanine, leucine 1 , leucine 2 , threonine, tryptophan, and valine tRNAs. On the basis of the anticodon sequences of t...
Publication Info
- Year
- 2001
- Type
- article
- Volume
- 25
- Issue
- 10
- Pages
- 963-969
- Citations
- 7
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
7
OpenAlex
Cite This
Charlotta Movitz,
Cláes Dahlgren
(2001).
QUANTIFICATION OF ANNEXIN I IN SUBCELLULAR FRACTIONS OF HUMAN NEUTROPHILS REVEALS AN EXCLUSIVE CYTOSOLIC LOCALISATION.
Cell Biology International
, 25
(10)
, 963-969.
https://doi.org/10.1006/cbir.2001.0760
Identifiers
- DOI
- 10.1006/cbir.2001.0760