Quantitative PCR and RT-PCR in virology.

Massimo Clementi; Stefano Menzo; Maria Carla Re; Aldo Manzin; A Valenza; Pietro E. Varaldo

doi:10.1101/gr.2.3.191

Abstract

In the last few years, molecular hybridization methods have been used extensively in basic and applied virology because of their technical flexibility and high specificity. Using these techniques, the detection of DNA and RNA viruses directly from clinical specimens, the analysis of the specific transcriptional activity of viral genes in vitro and in vivo, and the study of virus-host relationships have all been carried out at the molecular level. However, although these methods are efficient for many purposes, only development (~) and optimization (z) of PCR amplification have dramatically improved sensitivity. Currently, PCR is the method of choice for the detection of viral nucleic acids present at very low amounts in biological samples, and it allows the molecular study of most acute and persistent viral infections. Impressive achievements that are rap

Keywords

BiologyVirologyReal-time polymerase chain reactionPolymerase chain reactionComputational biologyGeneticsGene

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Publication Info

Year: 1993
Type: review
Volume: 2
Issue: 3
Pages: 191-196
Citations: 150
Access: Closed

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APA Style

                            
                                
                                    Massimo Clementi, 
                                
                                    Stefano Menzo, 
                                
                                    Maria Carla Re
                                
                                et al.
                            
                            (1993). 
                            Quantitative PCR and RT-PCR in virology.. 
                            Genome Research
                            , 2
                            (3)
                            , 191-196.
                            https://doi.org/10.1101/gr.2.3.191
                        

Identifiers

DOI: 10.1101/gr.2.3.191