Abstract
Highly luminescent semiconductor quantum dots (zinc sulfide–capped cadmium selenide) have been covalently coupled to biomolecules for use in ultrasensitive biological detection. In comparison with organic dyes such as rhodamine, this class of luminescent labels is 20 times as bright, 100 times as stable against photobleaching, and one-third as wide in spectral linewidth. These nanometer-sized conjugates are water-soluble and biocompatible. Quantum dots that were labeled with the protein transferrin underwent receptor-mediated endocytosis in cultured HeLa cells, and those dots that were labeled with immunomolecules recognized specific antibodies or antigens.
Keywords
Quantum dotPhotobleachingChemistryCadmium selenideLuminescenceNanotechnologyBiomoleculeZinc sulfideEndocytosisCadmium sulfideNanoparticleCovalent bondCyanineConjugateBioconjugationFluorescenceMaterials scienceZincCombinatorial chemistryOptoelectronicsInorganic chemistryBiochemistry
MeSH Terms
Cadmium CompoundsEndocytosisFluorescent Antibody TechniqueFluorescent DyesHeLa CellsHumansLuminescenceMolecular Probe TechniquesParticle SizeReceptorsTransferrinSelenium CompoundsSemiconductorsSolubilitySulfidesTransferrinZinc Compounds
Affiliated Institutions
Related Publications
Solar Energy Conversion by Dye-Sensitized Photovoltaic Cells
The quality of human life depends to a large degree on the availability of energy. This is threatened unless renewable energy resources can be developed in the near future. Chem...
Superresolution by localization of quantum dots using blinking statistics
In microscopy, single fluorescence point sources can be localized with a precision several times greater than the resolution limit of the microscope. We show that the intermitte...
Polarization-Selective Plasmon-Enhanced Silicon Quantum-Dot Luminescence
The photoluminescence intensity of silicon quantum dots is enhanced in a polarization-selective way by coupling to elongated Ag nanoparticles. The observed polarization dependen...
In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles
Fluorescent semiconductor nanocrystals (quantum dots) have the potential to revolutionize biological imaging, but their use has been limited by difficulties in obtaining nanocry...
Publication Info
- Year
- 1998
- Type
- article
- Volume
- 281
- Issue
- 5385
- Pages
- 2016-2018
- Citations
- 7206
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
7206
OpenAlex
81
Influential
6231
CrossRef
Cite This
Warren C. W. Chan,
Shuming Nie
(1998).
Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection.
Science
, 281
(5385)
, 2016-2018.
https://doi.org/10.1126/science.281.5385.2016
Identifiers
- DOI
- 10.1126/science.281.5385.2016
- PMID
- 9748158