Keywords
Thermus aquaticusPolymerase chain reactionPolymeraseReal-time polymerase chain reactionTemperature cyclingHot start PCRComputational biologyComputer scienceChemistryBiologyNested polymerase chain reactionBiochemistryThermalEnzymePhysicsThermodynamics
Affiliated Institutions
Related Publications
Taq DNA polymerase slippage mutation rates measured by PCR and quasi-likelihood analysis: (CA/GT)n and (A/T)n microsatellites
During microsatellite polymerase chain reaction (PCR), insertion-deletion mutations produce stutter products differing from the original template by multiples of the repeat unit...
Ligation-independent cloning of PCR products (LIC-PCR)
A new procedure has been developed for the efficient cloning of complex PCR mixtures, resulting in libraries exclusively consisting of recombinant clones. Recombinants are gener...
USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products
We present a method that allows simultaneous fusion and cloning of multiple PCR products in a rapid and efficient manner. The procedure is based on the use of PCR primers that c...
Gene Splicing by Overlap Extension: Tailor-Made Genes Using the Polymerase Chain Reaction
Gene Splicing by Overlap Extension or "gene SOEing" is a PCR-based method of recombining DNA sequences without reliance on restriction sites and of directly generating mutated D...
Cell-free cloning using φ29 DNA polymerase
We describe conditions for rolling-circle amplification (RCA) of individual DNA molecules 5–7 kb in size by >10 9 -fold, using φ29 DNA polymerase. The principal difficulty wi...
Publication Info
- Year
- 2001
- Type
- book-chapter
- Pages
- 1-8
- Citations
- 634
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
634
OpenAlex
Cite This
Carl T. Wittwer
(2001).
Rapid Cycle Real-Time PCR: Methods and Applications.
, 1-8.
https://doi.org/10.1007/978-3-642-59524-0_1
Identifiers
- DOI
- 10.1007/978-3-642-59524-0_1