Raptor, a Binding Partner of Target of Rapamycin (TOR), Mediates TOR Action

Kenta Hara; Yoshiko Maruki; Samuel Long; K. Yoshino; Noriko Oshiro; Hidayat Sujuti; Chiharu Tokunaga; Joseph Avruch; Kazuyoshi Yonezawa

doi:10.1016/s0092-8674(02)00833-4

Abstract

mTOR controls cell growth, in part by regulating p70 S6 kinase alpha (p70alpha) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). Raptor is a 150 kDa mTOR binding protein that also binds 4EBP1 and p70alpha. The binding of raptor to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70alpha. Rapamycin or amino acid withdrawal increases, whereas insulin strongly inhibits, the recovery of 4EBP1 and raptor on 7-methyl-GTP Sepharose. Partial inhibition of raptor expression by RNA interference (RNAi) reduces mTOR-catalyzed 4EBP1 phosphorylation in vitro. RNAi of C. elegans raptor yields an array of phenotypes that closely resemble those produced by inactivation of Ce-TOR. Thus, raptor is an essential scaffold for the mTOR-catalyzed phosphorylation of 4EBP1 and mediates TOR action in vivo.

Keywords

BiologyAction (physics)GeneticsCell biologyEvolutionary biology

MeSH Terms

Adaptor ProteinsSignal TransducingAnimalsBase SequenceCaenorhabditis elegansCarrier ProteinsCell Cycle ProteinsCell LineCloningMolecularDNAComplementaryEnzyme ActivationGene ExpressionGene SilencingHeLa CellsHumansMolecular Sequence DataPhenotypePhosphoproteinsPhosphorylationProtein BindingProtein KinasesProteinsRNA Cap AnalogsRNASmall InterferingRNAUntranslatedRegulatory-Associated Protein of mTORRibosomal Protein S6 KinasesSepharoseTOR Serine-Threonine Kinases

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Publication Info

Year: 2002
Type: article
Volume: 110
Issue: 2
Pages: 177-189
Citations: 1769
Access: Closed

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APA Style

                            
                                
                                    Kenta Hara, 
                                
                                    Yoshiko Maruki, 
                                
                                    Samuel Long
                                
                                et al.
                            
                            (2002). 
                            Raptor, a Binding Partner of Target of Rapamycin (TOR), Mediates TOR Action. 
                            Cell
                            , 110
                            (2)
                            , 177-189.
                            https://doi.org/10.1016/s0092-8674(02)00833-4
                        

Identifiers

DOI: 10.1016/s0092-8674(02)00833-4
PMID: 12150926

Data Quality

Data completeness: 86%