Abstract
The B19 parvovirus is responsible for at least three human diseases. The virus was successfully propagated in suspension cultures of human erythroid bone marrow from patients with hemolytic anemias; release of newly synthesized virus into the supernatants of infected cultures was observed. This culture system allowed study at a molecular level of events associated with the B19 life cycle. The B19 parvovirus replicated through high molecular weight intermediate forms, linked through a terminal hairpin structure. B19 replication in vitro was highly dependent on the erythropoietic content of cultures and on addition of the hormone erythropoietin.
Keywords
ParvovirusParvoviridaeErythropoietinVirologyVirusBone marrowBiologyIn vitroCell cultureViral replicationSuspension cultureErythropoiesisImmunologyAnemiaGeneticsMedicineInternal medicine
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Publication Info
- Year
- 1986
- Type
- article
- Volume
- 233
- Issue
- 4766
- Pages
- 883-886
- Citations
- 328
- Access
- Closed
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Cite This
Keiya Ozawa,
Gary J. Kurtzman,
Neal S. Young
(1986).
Replication of the B19 Parvovirus in Human Bone Marrow Cell Cultures.
Science
, 233
(4766)
, 883-886.
https://doi.org/10.1126/science.3738514
Identifiers
- DOI
- 10.1126/science.3738514