Abstract

Stimulation of rat vascular smooth muscle cells (VSMCs) by platelet-derived growth factor (PDGF) transiently increased the intracellular concentration of hydrogen peroxide (H 2 O 2 ). This increase could be blunted by increasing the intracellular concentration of the scavenging enzyme catalase or by the chemical antioxidant N -acetylcysteine. The response of VSMCs to PDGF, which includes tyrosine phosphorylation, mitogen-activated protein kinase stimulation, DNA synthesis, and chemotaxis, was inhibited when the growth factor-stimulated rise in H 2 O 2 concentration was blocked. These results suggest that H 2 O 2 may act as a signal-transducing molecule, and they suggest a potential mechanism for the cardioprotective effects of antioxidants.

Keywords

CatalaseIntracellularPlatelet-derived growth factor receptorHydrogen peroxideSignal transductionGrowth factorStimulationVascular smooth muscleCell biologyPlatelet-derived growth factorBiochemistryDNA synthesisPhosphorylationChemistryChemotaxisProtein kinase AAntioxidantBiologyEndocrinologyDNAReceptorSmooth muscle

Affiliated Institutions

Related Publications

Publication Info

Year
1995
Type
article
Volume
270
Issue
5234
Pages
296-299
Citations
2515
Access
Closed

External Links

View on DOI.org

Social Impact

Altmetric
PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

2515
OpenAlex

Cite This

Maitrayee Sundaresan, Zu-Xi Yu, Victor J. Ferrans et al. (1995). Requirement for Generation of H <sub>2</sub> O <sub>2</sub> for Platelet-Derived Growth Factor Signal Transduction. Science , 270 (5234) , 296-299. https://doi.org/10.1126/science.270.5234.296

Identifiers

DOI
10.1126/science.270.5234.296