Abstract

A P-element vector has been constructed and used to generate lines of flies with single autosomal P-element insertions. The lines were analyzed in two ways: (1) the identification of cis-acting patterning information within the Drosophila genome, as revealed by a lacZ reporter gene within the P element, and (2) the isolation of lethal mutations. We examined 3768 independent lines for the expression of lacZ in embryos and looked among these lines for lethal mutations affecting embryonic neurogenesis. This type of screen appears to be an effective way to find new loci that may play a role in the development of the Drosophila nervous system.

Keywords

BiologyGeneticslac operonGenomeGeneEmbryonic stem cellMutationReporter geneDrosophila (subgenus)DrosophilidaeDrosophila melanogasterGene expression

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Publication Info

Year
1989
Type
article
Volume
3
Issue
9
Pages
1273-1287
Citations
684
Access
Closed

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Ethan Bier, Harald Vaessin, S Shepherd et al. (1989). Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector.. Genes & Development , 3 (9) , 1273-1287. https://doi.org/10.1101/gad.3.9.1273

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DOI
10.1101/gad.3.9.1273