Abstract
We demonstrate a novel all-optical switch consisting of two molecules: a primary fluorophore (Cy5) that can be switched between a fluorescent and a dark state by light of different wavelengths, and a secondary chromophore (Cy3) that facilitates switching. The interaction between the two molecules exhibits a distance dependence much steeper than that of conventional Fo rster resonance energy transfer. This enables the switch to act as a ruler with the capability to probe distances difficult to access by other spectroscopic methods, thus presenting a new tool for the study of biomolecules at the single-molecule level.
Keywords
FluorophoreChromophoreFörster resonance energy transferMoleculeMolecular switchFluorescenceBiomoleculeMaterials scienceWavelengthRange (aeronautics)Resonance (particle physics)Optical switchOptoelectronicsNanotechnologyMolecular physicsOpticsAtomic physicsPhysicsChemistryPhotochemistry
MeSH Terms
Bacterial ProteinsBiotinCarbocyaninesDNADNA DamageFluorescence Resonance Energy TransferModelsMolecularOptics and PhotonicsRNACatalyticSerum AlbuminBovine
Affiliated Institutions
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Publication Info
- Year
- 2005
- Type
- article
- Volume
- 94
- Issue
- 10
- Pages
- 108101-108101
- Citations
- 361
- Access
- Closed
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Cite This
Mark Bates,
Timothy R. Blosser,
Xiaowei Zhuang
(2005).
Short-Range Spectroscopic Ruler Based on a Single-Molecule Optical Switch.
Physical Review Letters
, 94
(10)
, 108101-108101.
https://doi.org/10.1103/physrevlett.94.108101
Identifiers
- DOI
- 10.1103/physrevlett.94.108101
- PMID
- 15783528
- PMCID
- PMC2652517
- arXiv
- q-bio/0502012