Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris

The Tabula Muris Consortium; Overall coordination; Logistical coordination; Cell type annotation; Writing Group; Principal Investigators

doi:10.1038/s41586-018-0590-4

Abstract

Here we present a compendium of single-cell transcriptomic data from the model organism Mus musculus that comprises more than 100,000 cells from 20 organs and tissues. These data represent a new resource for cell biology, reveal gene expression in poorly characterized cell populations and enable the direct and controlled comparison of gene expression in cell types that are shared between tissues, such as T lymphocytes and endothelial cells from different anatomical locations. Two distinct technical approaches were used for most organs: one approach, microfluidic droplet-based 3'-end counting, enabled the survey of thousands of cells at relatively low coverage, whereas the other, full-length transcript analysis based on fluorescence-activated cell sorting, enabled the characterization of cell types with high sensitivity and coverage. The cumulative data provide the foundation for an atlas of transcriptomic cell biology.

Keywords

TranscriptomeBiologyCell sortingCellCell typeCell biologyComputational biologyGene expressionGeneGenetics

MeSH Terms

AnimalsCell BiologyEndothelial CellsFemaleFlow CytometryGene Expression ProfilingMaleMiceMiceInbred C57BLMicrofluidicsOrgan SpecificitySingle-Cell AnalysisT-LymphocytesTranscription FactorsTranscriptome

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Publication Info

Year: 2018
Type: article
Volume: 562
Issue: 7727
Pages: 367-372
Citations: 2962
Access: Closed

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APA Style

                            
                                
                                    The Tabula Muris Consortium, 
                                
                                    Overall coordination, 
                                
                                    Logistical coordination
                                
                                et al.
                            
                            (2018). 
                            Single-cell transcriptomics of 20 mouse organs creates a Tabula Muris. 
                            Nature
                            , 562
                            (7727)
                            , 367-372.
                            https://doi.org/10.1038/s41586-018-0590-4
                        

Identifiers

DOI: 10.1038/s41586-018-0590-4
PMID: 30283141
PMCID: PMC6642641

Data Quality

Data completeness: 86%