Abstract

Toxins from scorpion venom interact with potassium channels. Resin-attached, mutant K + channels from Streptomyces lividans were used to screen venom from Leiurus quinquestriatus hebraeus , and the toxins that interacted with the channel were rapidly identified by mass spectrometry. One of the toxins, agitoxin2, was further studied by mutagenesis and radioligand binding. The results show that a prokaryotic K + channel has the same pore structure as eukaryotic K + channels. This structural conservation, through application of techniques presented here, offers a new approach for K + channel pharmacology.

Keywords

VenomPotassium channelMutagenesisMutantBiophysicsChemistryBiologyBiochemistryComputational biologyGene

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Publication Info

Year
1998
Type
article
Volume
280
Issue
5360
Pages
106-109
Citations
403
Access
Closed

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Roderick MacKinnon, Steven L. Cohen, Anling Kuo et al. (1998). Structural Conservation in Prokaryotic and Eukaryotic Potassium Channels. Science , 280 (5360) , 106-109. https://doi.org/10.1126/science.280.5360.106

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DOI
10.1126/science.280.5360.106