Abstract
We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.
Keywords
FluorophoreMicroscopyResolution (logic)Photoactivated localization microscopyFluorescence-lifetime imaging microscopyOpticsFluorescence microscopeDiffractionFluorescenceMaterials scienceImage resolutionSuper-resolution microscopyPhysicsComputer scienceArtificial intelligence
MeSH Terms
DNAFluorescent DyesImage InterpretationComputer-AssistedMicroscopyFluorescenceNanotechnologyStochastic Processes
Affiliated Institutions
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Publication Info
- Year
- 2006
- Type
- article
- Volume
- 3
- Issue
- 10
- Pages
- 793-796
- Citations
- 8018
- Access
- Closed
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Cite This
Michael J. Rust,
Mark Bates,
Xiaowei Zhuang
(2006).
Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).
Nature Methods
, 3
(10)
, 793-796.
https://doi.org/10.1038/nmeth929
Identifiers
- DOI
- 10.1038/nmeth929
- PMID
- 16896339
- PMCID
- PMC2700296