Abstract
The existence of two sophisticated parallel splicing machineries in multicellular organisms has raised intriguing questions--ranging from their impact on proteome expansion to the evolution of splicing and of metazoan genomes. Exploring roles for the distinct splicing systems in vivo has, however, been restricted by the lack of techniques to selectively inhibit their function in cells. In this study, we show that morpholino oligomers complementary to the branch-site recognition elements of U2 or U12 small nuclear RNA specifically suppress the function of the two splicing systems in mammalian cells. The data provide the first evidence for a role of distinct spliceosomes in pre-mRNA splicing from endogenous mammalian genes and establish a tool to define roles for the different splicing machineries in vivo.
Keywords
BiologySpliceosomeRNA splicingProteomeGene knockdownMulticellular organismCell biologyPrp24Splicing factorAlternative splicingGeneticsMinigeneComputational biologyPrecursor mRNAExonic splicing enhancerMorpholinoFunction (biology)IntronRNAGeneMessenger RNA
Affiliated Institutions
Related Publications
Systematic Profiling of Poly(A)+ Transcripts Modulated by Core 3’ End Processing and Splicing Factors Reveals Regulatory Rules of Alternative Cleavage and Polyadenylation
Alternative cleavage and polyadenylation (APA) results in mRNA isoforms containing different 3' untranslated regions (3'UTRs) and/or coding sequences. How core cleavage/polyaden...
VIRMA mediates preferential m6A mRNA methylation in 3′UTR and near stop codon and associates with alternative polyadenylation
N6-methyladenosine (m6A) is enriched in 3′untranslated region (3′UTR) and near stop codon of mature polyadenylated mRNAs in mammalian systems and has regulatory roles in eukaryo...
Predictive Identification of Exonic Splicing Enhancers in Human Genes
Specific short oligonucleotide sequences that enhance pre-mRNA splicing when present in exons, termed exonic splicing enhancers (ESEs), play important roles in constitutive and ...
U1 snRNP regulates cancer cell migration and invasion in vitro
Abstract Stimulated cells and cancer cells have widespread shortening of mRNA 3’-untranslated regions (3’UTRs) and switches to shorter mRNA isoforms due to usage of more proxima...
Depletion of TDP 43 overrides the need for exonic and intronic splicing enhancers in the human apoA-II gene
Exon 3 of the human apolipoprotein A-II (apoA-II) gene is efficiently included in the mRNA although its acceptor site is significantly weak because of a peculiar (GU)16 tract in...
Publication Info
- Year
- 2005
- Type
- article
- Volume
- 33
- Issue
- 4
- Pages
- e41-e41
- Citations
- 26
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
26
OpenAlex
Cite This
Nathalie Matter
(2005).
Targeted 'knockdown' of spliceosome function in mammalian cells.
Nucleic Acids Research
, 33
(4)
, e41-e41.
https://doi.org/10.1093/nar/gni041
Identifiers
- DOI
- 10.1093/nar/gni041