Abstract
The 220 3'-terminal nucleotides of the small ribosomal subunit RNA (13S) of hamster (BHK-21) cell mitochondria have been sequenced and the positions of post-transcriptionally methylated residues within this sequence have been established. Also, we have derived the secondary structure of the 3'-terminus of mitochondrial 13S rRNA by 1) searching nucleotide sequences of 13S rRNA, procaryotic 16S rRNA and eucaryotic 18S rRNA for common secondary structures and 2) using single-strand specific endonucleases to map secondary interactions in 13S rRNA. The pyrimidine tract CCUCC in E. coli 16S rRNA, which participates in base-pairing with bacterial mRNA, is absent in mitochondrial 13S rRNA. We believe that the binding of mRNA to mammalian mitochondrial ribosomes is not mediated by a conventional Shine-Dalgarno interaction.
Keywords
BiologyRibosomal RNARibosome18S ribosomal RNAMitochondrial ribosomeMolecular biologyEukaryotic RibosomeNucleic acid sequence5.8S ribosomal RNARNAGeneticsGene
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Publication Info
- Year
- 1980
- Type
- article
- Volume
- 8
- Issue
- 21
- Pages
- 4927-4941
- Citations
- 46
- Access
- Closed
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Cite This
Richard Baer,
Donald T. Dubin
(1980).
The 3′-terminal sequence of the small subunit ribosomal RNA from hamster mitochondria.
Nucleic Acids Research
, 8
(21)
, 4927-4941.
https://doi.org/10.1093/nar/8.21.4927
Identifiers
- DOI
- 10.1093/nar/8.21.4927