Abstract
A procedure for the hydrolysis of phospholipids is described. The effect of alkali concentration, organic solvents, and length of hydrolysis have been studied. The initial reaction in the preferred solvent system appears to be a methanolysis. The phospholipids are converted quantitatively into water-soluble phosphates, which are separated and estimated by ion-exchange chromatography. In this way phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and phosphatidylinositol may be determined in liver tissue.
Keywords
HydrolysisChemistryPig liverBiochemistryChromatographyLiver tissueBiologyEnzymeEndocrinology
Affiliated Institutions
Related Publications
Cooperative roles of various membrane phospholipids in the activation of calcium-activated, phospholipid-dependent protein kinase.
Although phosphatidylserine is the sole phospholipid effective for the activation of Ca2+-activated, phospholipid-dependent protein kinase in the presence of a small amount of u...
Ectocytosis caused by sublytic autologous complement attack on human neutrophils. The sorting of endogenous plasma-membrane proteins and lipids into shed vesicles
During sublytic complement attack on human neutrophils, plasma-membrane vesicles are shed from the cell surface as a cell-protection mechanism. By using surface-iodinated neutro...
Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.
The proenzyme of a Ca2+-dependent protease-activated protein kinase previously obtained from mammalian tissues (Inoue, M., Kishimoto, A., Takai, Y., and Nishizuka, Y. (1977) J. ...
Phosphorylation of tau proteins to a state like that in Alzheimer's brain is catalyzed by a calcium/calmodulin-dependent kinase and modulated by phospholipids.
Calcium/calmodulin (CaM)-dependent protein kinases isolated from bovine and rat brains phosphorylate the microtubule-associated tau protein in the mode that shifts the mobility ...
Production of diglyceride from phosphatidylinositol in activated human platelets.
Human platelets generate diglyceride within 5 s of exposure to thrombin. Production of diglyceride is transient. 15 s after the addition of thrombin, the levels of diglyceride h...
Publication Info
- Year
- 1960
- Type
- article
- Volume
- 1
- Issue
- 5
- Pages
- 433-438
- Citations
- 126
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
126
OpenAlex
Cite This
G. Hübscher,
J. N. Hawthorne,
P. Kemp
(1960).
The analysis of tissue phospholipids: hydrolysis procedure and results with pig liver.
Journal of Lipid Research
, 1
(5)
, 433-438.
https://doi.org/10.1016/s0022-2275(20)41229-5
Identifiers
- DOI
- 10.1016/s0022-2275(20)41229-5