Abstract
lin-4 is essential for the normal temporal control of diverse postembryonic developmental events in C. elegans. lin-4 acts by negatively regulating the level of LIN-14 protein, creating a temporal decrease in LIN-14 protein starting in the first larval stage (L1). We have cloned the C. elegans lin-4 locus by chromosomal walking and transformation rescue. We used the C. elegans clone to isolate the gene from three other Caenorhabditis species; all four Caenorhabditis clones functionally rescue the lin-4 null allele of C. elegans. Comparison of the lin-4 genomic sequence from these four species and site-directed mutagenesis of potential open reading frames indicated that lin-4 does not encode a protein. Two small lin-4 transcripts of approximately 22 and 61 nt were identified in C. elegans and found to contain sequences complementary to a repeated sequence element in the 3' untranslated region (UTR) of lin-14 mRNA, suggesting that lin-4 regulates lin-14 translation via an antisense RNA-RNA interaction.
Keywords
BiologyCaenorhabditis elegansGeneticsGeneUntranslated regionCaenorhabditisAntisense RNAOpen reading frameRNAPeptide sequence
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Publication Info
- Year
- 1993
- Type
- article
- Volume
- 75
- Issue
- 5
- Pages
- 843-854
- Citations
- 12649
- Access
- Closed
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Cite This
Rosalind C. Lee,
Rhonda Feinbaum,
Victor Ambros
(1993).
The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14.
Cell
, 75
(5)
, 843-854.
https://doi.org/10.1016/0092-8674(93)90529-y
Identifiers
- DOI
- 10.1016/0092-8674(93)90529-y