Abstract
The coupling of the tetrazolium salts triphenyltetrazolium chloride and nitro-blue tetrazolium to the electron transport chain in mitochondria of thymus, spleen, liver, kidney, and Ehrlich ascites carcinoma cells has been studied with several substrates. In experiments on succinate–triphenyltetrazolium chloride reductase activity it has been possible to demonstrate a radiation lesion in the electron transport chain of mitochondria from thymus and spleen, but not in those from other tissues. This lesion is evident 4 hours after 25 rad of whole-body irradiation, or earlier with higher doses. It is not prevented by the prior administration of aminoethylisothiouronium bromide, serotonin, vitamin K 1 , or vitamin E, but is reduced by anoxic conditions.Lower levels of cytochrome c are found in irradiated mitochondria isolated from thymus, and the radiation lesion is believed to be produced by loosening the binding of cytochrome c to the mitochondrial membrane after X-irradiation. Decreased levels of ATP occur in thymus, spleen, and ascites cells following irradiation.
Keywords
MitochondrionCytochrome cSpleenChemistryLesionEnzymeBiochemistryBiologyMolecular biologyMedicinePathologyImmunology
MeSH Terms
AnimalsCytochromesElectron Transport Complex IVFemaleMitochondriaRadiation EffectsRats
Related Publications
Mitochondrial cytochrome c release in apoptosis occurs upstream of DEVD-specific caspase activation and independently of mitochondrial transmembrane depolarization
Mitochondrial cytochrome c, which functions as an electron carrier in the respiratory chain, translocates to the cytosol in cells undergoing apoptosis, where it participates in ...
Mitochondria and Apoptosis
REVIEW A variety of key events in apoptosis focus on mitochondria, including the release of caspase activators (such as cytochrome c), changes in electron transport, loss of mit...
Bax directly induces release of cytochrome <i>c</i> from isolated mitochondria
Bax is a pro-apoptotic member of the Bcl-2 protein family that resides in the outer mitochondrial membrane. It is controversial whether Bax promotes cell death directly through ...
Cybrids in Alzheimer's disease: A cellular model of the disease?
The mitochondrial electron transport chain enzyme cytochrome c oxidase (COX) is defective in patients with sporadic Alzheimer's disease (AD). This defect arises from the mutatio...
Five TGA “stop” codons occur within the translated sequence of the yeast mitochondrial gene for cytochrome <i>c</i> oxidase subunit II
A mitochondrial mutation that genetically maps in the middle of the gene coding cytochrome c oxidase subunit II has been found to be a single-base-pair deletion. Three independe...
Publication Info
- Year
- 1966
- Type
- article
- Volume
- 44
- Issue
- 4
- Pages
- 433-448
- Citations
- 22
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
22
OpenAlex
1
Influential
16
CrossRef
Cite This
J. F. Scaife
(1966).
THE EFFECT OF LETHAL DOSES OF X-IRRADIATION ON THE ENZYMATIC ACTIVITY OF MITOCHONDRIAL CYTOCHROME<i>c</i>.
Canadian Journal of Biochemistry
, 44
(4)
, 433-448.
https://doi.org/10.1139/o66-053
Identifiers
- DOI
- 10.1139/o66-053
- PMID
- 4289628