Abstract
The early stages of absorption of intravenously injected horseradish peroxidase in proximal tubules of mouse kidney were studied with a new ultrastructural cytochemical technique. In animals killed as early as 90 sec after injection, reaction product was found on the brushborder membranes and in the apical tubular invaginations. From the latter structures it was transported to the apical vacuoles, in which it was progressively concentrated to form protein absorption droplets. The method, which employs 3,3'-diaminobenzidine as oxidizable substrate, gives sharp localization and is sensitive. This system is advantageous in studying the early stages of renal tubular protein absorption, since small amounts of protein on membranes and in tubules and vesicles can be detected easily. The method also appears promising for studying protein transport in a variety of other cells and tissues.
Keywords
Horseradish peroxidaseCytochemistryUltrastructureVacuolePeroxidaseVesicleChemistryAbsorption (acoustics)KidneyMembraneBiophysicsBiochemistryBiologyAnatomyEnzymeMaterials scienceCytoplasmEndocrinology
Affiliated Institutions
Related Publications
HEMOGLOBIN ABSORPTION BY THE CELLS OF THE PROXIMAL CONVOLUTED TUBULE IN MOUSE KIDNEY
The formation of protein absorption droplets in the cells of the proximal convolution was studied in mouse kidney. Ox hemoglobin was administered intraperitoneally and kidney sp...
EXPERIMENTAL STUDIES IN ACUTE RENAL FAILURE
When purified human globin is injected intravenously into rats it produces acute renal failure characterized by tubular casts and oliguria. The globin is identifiable within ves...
FACTORS AFFECTING THE STATE OF INJECTED HORSERADISH PEROXIDASE IN ANIMAL TISSUES AND PROCEDURES FOR THE STUDY OF PHAGOSOMES AND PHAGO-LYSOSOMES
It was attempted to find conditions at which the localization of injected horseradish peroxidase was preserved at its original sites and artifacts of diffusion and unspecific ad...
Application of the Electron Microscope to the Cytochemical Peroxidase Reaction in Salamander Leukocytes
The present study has dealt with the localization by electron microscopy of the products of peroxidase reaction in neutrophil leukocytes in the subcapsular region of the livers ...
Caveolin-3 Associates with Developing T-tubules during Muscle Differentiation
Caveolae, flask-shaped invaginations of the plasma membrane, are particularly abundant in muscle cells. We have recently cloned a muscle-specific caveolin, termed caveolin-3, wh...
Publication Info
- Year
- 1966
- Type
- article
- Volume
- 14
- Issue
- 4
- Pages
- 291-302
- Citations
- 6504
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
6504
OpenAlex
Cite This
Richard C. Graham,
Morris J. Karnovsky
(1966).
THF EARLY STAGES OF ABSORPTION OF INJECTED HORSERADISH PEROXIDASE IN THE PROXIMAL TUBULES OF MOUSE KIDNEY: ULTRASTRUCTURAL CYTOCHEMISTRY BY A NEW TECHNIQUE.
Journal of Histochemistry & Cytochemistry
, 14
(4)
, 291-302.
https://doi.org/10.1177/14.4.291
Identifiers
- DOI
- 10.1177/14.4.291