Abstract

Drosophila Toll protein is shown to activate the innate immune system in adult and regulate the dorsoventral patterning in the developing embryo. Recently, five human homologs of Drosophila Toll, designated as Toll-like receptors (TLRs), have been identified and shown to play a role in the innate immune response. We report here the molecular cloning and characterization of a new member of Toll-like receptor family, Toll-like receptor 6 (TLR6). Human and murine TLR6 are type-I transmembrane receptors that contain both an extracellular leucine-rich repeat (LRR) domain and a cytoplasmic Toll/IL-1 receptor (IL-1R)-like region. The amino acid sequence of human TLR6 (hTLR6) is most similar to that of hTLR1 with 69% identity. RT-PCR analysis revealed that murine TLR6 is expressed predominantly in spleen, thymus, ovary and lung. Like other TLR family members, constitutively active TLR6 activates both NF-kappaB and c-Jun N-terminal kinase (JNK). The TLR6 gene, as well as the TLR1 gene, mapped to the proximal region of murine chromosome 5 within 1.7cM of each other. These results suggest that TLR6 is a novel member of an expanding TLR family.

Keywords

BiologyInnate immune systemReceptorToll-like receptorTransmembrane proteinTransmembrane domainGeneGene familyCell biologyImmune systemGeneticsGene expression

MeSH Terms

3T3 CellsAmino Acid SequenceAnimalsBase SequenceCalcium-Calmodulin-Dependent Protein KinasesChromosome MappingCloningMolecularCrossesGeneticDNAComplementaryDrosophilaDrosophila ProteinsEnzyme ActivationHumansJNK Mitogen-Activated Protein KinasesMembrane GlycoproteinsMiceMiceInbred C57BLMitogen-Activated Protein KinasesMolecular Sequence DataNF-kappa BRNAMessengerReceptorsCell SurfaceSequence HomologyAmino AcidToll-Like Receptor 1Toll-Like Receptor 6Toll-Like Receptors

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Publication Info

Year
1999
Type
article
Volume
231
Issue
1-2
Pages
59-65
Citations
430
Access
Closed

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Cite This

Osamu Takeuchi, Taro Kawai, Hideki Sanjo et al. (1999). TLR6: A novel member of an expanding Toll-like receptor family. Gene , 231 (1-2) , 59-65. https://doi.org/10.1016/s0378-1119(99)00098-0

Identifiers

DOI
10.1016/s0378-1119(99)00098-0
PMID
10231569

Data Quality

Data completeness: 81%