Unlocking Short Read Sequencing for Metagenomics

Abstract

This strategy is broadly applicable to sequencing applications that benefit from low-cost high-throughput sequencing, but require longer read lengths. We demonstrate that our approach enables metagenomic analyses using the Illumina Genome Analyzer, with low error rates, and at a fraction of the cost of pyrosequencing.

Keywords

MetagenomicsSanger sequencingDNA sequencingComputational biologyPyrosequencingComputer sciencePipeline (software)Shotgun sequencingSoftwareDeep sequencingBiologyGenomeData miningGeneticsDNAGene

Affiliated Institutions

Massachusetts Institute of Technology US

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Publication Info

Year: 2010
Type: article
Volume: 5
Issue: 7
Pages: e11840-e11840
Citations: 167
Access: Closed

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APA Style

                            
                                
                                    Sébastien Rodrigue, 
                                
                                    Arne Materna, 
                                
                                    Sonia Timberlake
                                
                                et al.
                            
                            (2010). 
                            Unlocking Short Read Sequencing for Metagenomics. 
                            PLoS ONE
                            , 5
                            (7)
                            , e11840-e11840.
                            https://doi.org/10.1371/journal.pone.0011840
                        

Identifiers

DOI: 10.1371/journal.pone.0011840