Keywords
Calibration curveReal-time polymerase chain reactionBiologyReproducibilityCalibrationReverse transcriptaseComplementary DNAMolecular biologyReverse transcription polymerase chain reactionRNARecombinant DNAMessenger RNAStandard curveCoefficient of variationChemistryChromatographyDetection limitGeneMathematicsBiochemistryStatistics
Affiliated Institutions
Related Publications
Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays
The reverse transcription polymerase chain reaction (RT-PCR) is the most sensitive method for the detection of low-abundance mRNA, often obtained from limited tissue samples. Ho...
A novel method for real time quantitative RT-PCR.
A novel approach to quantitative reverse transcriptase polymerase chain reaction (QC RT-PCR) using real time detection and the 5' nuclease assay has been developed. Cystic fibro...
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems
The fluorescence-based real-time reverse transcription PCR (RT-PCR) is widely used for the quantification of steady-state mRNA levels and is a critical tool for basic research, ...
Relative expression software tool (REST(C)) for group-wise comparison and statistical analysis of relative expression results in real-time PCR
Real-time reverse transcription followed by polymerase chain reaction (RT-PCR) is the most suitable method for the detection and quantification of mRNA. It offers high sensitivi...
Specific detection by real-time reverse-transcription PCR assays of a novel avian influenza A(H7N9) strain associated with human spillover infections in China
In response to a recent outbreak in China, detection assays for a novel avian influenza A(H7N9) virus need to be implemented in a large number of public health laboratories. Her...
Publication Info
- Year
- 2001
- Type
- article
- Volume
- 23
- Issue
- 4
- Pages
- 275-282
- Citations
- 296
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
296
OpenAlex
Cite This
Michael W. Pfaffl,
Melanie Hageleit
(2001).
Validities of mRNA quantification using recombinant RNA and recombinant DNA external calibration curves in real-time RT-PCR.
Biotechnology Letters
, 23
(4)
, 275-282.
https://doi.org/10.1023/a:1005658330108
Identifiers
- DOI
- 10.1023/a:1005658330108