Abstract

We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high-quality sequence data without the need for target amplification and thus avoided concomitant biases.

Keywords

Terminator (solar)DNA sequencingNucleotideComputational biologyDNABiologySequencing by ligationGeneticsBase sequenceGeneGenomic libraryPhysics

MeSH Terms

AnimalsChromatographyHigh Pressure LiquidChromosomesArtificialBacterialComputer SimulationDNADogsNucleotidesSensitivity and SpecificitySequence AnalysisDNASubstrate Specificity

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Publication Info

Year
2009
Type
article
Volume
6
Issue
8
Pages
593-595
Citations
138
Access
Closed

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Cite This

Jayson Bowers, Judith Mitchell, E. George Beer et al. (2009). Virtual terminator nucleotides for next-generation DNA sequencing. Nature Methods , 6 (8) , 593-595. https://doi.org/10.1038/nmeth.1354

Identifiers

DOI
10.1038/nmeth.1354
PMID
19620973
PMCID
PMC2719685

Data Quality

Data completeness: 90%