Abstract

MicroRNAs (miRNAs) comprise 1 to 3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. By using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target messenger RNA molecules (mRNAs). Most targets are maternally expressed mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis.

Keywords

ZebrafishBiologymicroRNAMaternal to zygotic transitionCell biologyMessenger RNAMorphogenesisRegulation of gene expressionTranscriptomeRNAEmbryogenesisGene expressionTranscription (linguistics)GeneGeneticsEmbryoZygote

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Publication Info

Year
2006
Type
article
Volume
312
Issue
5770
Pages
75-79
Citations
1615
Access
Closed

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Cite This

Antonio J. Giráldez, Yuichiro Mishima, Jason Rihel et al. (2006). Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs. Science , 312 (5770) , 75-79. https://doi.org/10.1126/science.1122689

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DOI
10.1126/science.1122689