Alexander Varshavsky

The N-End Rule in Bacteria

The N-end rule relates the in vivo half-life of a protein to the identity of its amino-terminal residue. Distinct versions of the N-end rule operate in all eukaryotes examined. ...

Universality and Structure of the N-end Rule

The N-end rule is mediated by the UBC2(RAD6) ubiquitin-conjugating enzyme.

The N-end rule relates the in vivo half-life of a protein to the identity of its amino-terminal residue. Distinct versions of the N-end rule operate in all organisms examined, f...

Discovering the RNA Double Helix and Hybridization

In Vivo Half-Life of a Protein Is a Function of Its Amino-Terminal Residue

When a chimeric gene encoding a ubiquitin-β-galactosidase fusion protein is expressed in the yeast Saccharomyces cerevisiae , ubiquitin is cleaved off the nascent fusion protein...