Jacek R. Wiśniewski

Universal sample preparation method for proteome analysis

Proteomic Mapping of Brain Plasma Membrane Proteins

Proteomics is potentially a powerful technology for elucidating brain function and neurodegenerative diseases. So far, the brain proteome has generally been analyzed by two-dime...

Detergent-Based but Gel-Free Method Allows Identification of Several Hundred Membrane Proteins in Single LC-MS Runs

Detergents are indispensable solubilizing agents in the purification and analysis of membrane proteins. For mass spectrometric identification of proteins, it is essential that d...

HysTag—A Novel Proteomic Quantification Tool Applied to Differential Display Analysis of Membrane Proteins From Distinct Areas of Mouse Brain

A novel isotopically labeled cysteine-tagging and complexity-reducing reagent, called HysTag, has been synthesized and used for quantitative proteomics of proteins from enriched...

Analysis of the Mouse Liver Proteome Using Advanced Mass Spectrometry

We report a large-scale analysis of mouse liver tissue comprising a novel fractionation approach and high-accuracy mass spectrometry techniques. Two fractions enriched for solub...

Differential Analysis of Membrane Proteins in Mouse Fore- and Hindbrain Using a Label-Free Approach

The ability to quantitatively compare protein levels across different regions of the brain to identify disease mechanisms remains a fundamental research challenge. It requires b...

Deep proteome and transcriptome mapping of a human cancer cell line

While the number and identity of proteins expressed in a single human cell type is currently unknown, this fundamental question can be addressed by advanced mass spectrometry (M...

A “Proteomic Ruler” for Protein Copy Number and Concentration Estimation without Spike-in Standards

Absolute protein quantification using mass spectrometry (MS)-based proteomics delivers protein concentrations or copy numbers per cell. Existing methodologies typically require ...

Super-SILAC mix for quantitative proteomics of human tumor tissue