Absolute mRNA quantification using the polymerase chain reaction (PCR). A novel approach by a<i>P</i>CR aided<i>t</i>ranscipt<i>t</i>itration assay (PATTY)
The polymerase chain reaction (PCR) is used as part of a new approach to the absolute quantification of mRNA. We describe a PCR aided transcript titration assay (PATTY) which is...