Stefan W. Hell

STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis

Breaking the diffraction barrier in fluorescence microscopy at low light intensities by using reversibly photoswitchable proteins

Fluorescence microscopy is indispensable in many areas of science, but until recently, diffraction has limited the resolution of its lens-based variant. The diffraction barrier ...

Toward fluorescence nanoscopy

For more than a century, the resolution of focusing light microscopy has been limited by diffraction to 180 nm in the focal plane and to 500 nm along the optic axis. Recently, m...

Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy

We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emiss...

Improvement of lateral resolution in far-field fluorescence light microscopy by using two-photon excitation with offset beams