Abstract
A phage lambda cloning vector has been constructed which contains a single site for the restriction endonuclease BamHI. Since Sau3A and Bg/II produce the same cohesive ends as BamHI, this vector can also be used to clone DNA fragments generated with either of these enzymes. We have used this vector to construct an Escherichia coli library using partial digestion with Sau3A. This vector will be most useful for applications requiring genetic analysis of cloned E. coli genes.
Keywords
BiologyBamHIMultiple cloning siteCloning (programming)Restriction enzymeVector (molecular biology)Cloning vectorGeneticsMolecular cloningBacteriophageLibraryRestriction siteGenomic libraryMolecular biologyEscherichia coliVirologyRecombinant DNAGeneBase sequencePeptide sequenceComputer science
MeSH Terms
Bacteriophage lambdaBase SequenceCloningMolecularDNA Restriction EnzymesDNABacterialDNAViralEscherichia coliGenetic Vectors
Affiliated Institutions
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Publication Info
- Year
- 1982
- Type
- article
- Volume
- 20
- Issue
- 3
- Pages
- 317-322
- Citations
- 79
- Access
- Closed
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Cite This
Saeko Mizusawa,
D. Ward
(1982).
A bacteriophage lambda vector for cloning with BamHI and Sau3A.
Gene
, 20
(3)
, 317-322.
https://doi.org/10.1016/0378-1119(82)90200-1
Identifiers
- DOI
- 10.1016/0378-1119(82)90200-1
- PMID
- 6299896