Abstract

Abstract We demonstrate successful, simultaneous polymerase chain reaction (PCR) amplification of up to 300 000 discrete reactions in a novel platform, the PicoTiterPlate™. In addition to elevated throughput, the PicoTiterPlate™ based amplifications (PTPCR) can be performed in extremely small volumes: individual reactions volumes are as low as 39.5 pL, with a total 15.3 μL reaction volume for the entire PicoTiterPlate™. The bulk PTPCR product can be recovered and assayed with real‐time PCR, or discrete PTPCR products can be driven to solid supports, enabling downstream applications such as translation/transcription or sequencing.

Keywords

Polymerase chain reactionMassively parallelChemistryThroughputMassive parallel sequencingChain reactionPolymeraseComputational biologyNanotechnologyBiological systemComputer scienceBiologyMaterials scienceDNADNA sequencingGeneBiochemistryParallel computing

MeSH Terms

Base SequenceDNADNA PrimersFiber Optic TechnologyMicroscopyElectronScanningMiniaturizationNucleic Acid HybridizationPolymerase Chain ReactionSensitivity and Specificity

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Publication Info

Year
2003
Type
article
Volume
24
Issue
21
Pages
3769-3777
Citations
142
Access
Closed

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Cite This

John H. Leamon, William L. Lee, Karrie Tartaro et al. (2003). A massively parallel PicoTiterPlate™ based platform for discrete picoliter‐scale polymerase chain reactions. Electrophoresis , 24 (21) , 3769-3777. https://doi.org/10.1002/elps.200305646

Identifiers

DOI
10.1002/elps.200305646
PMID
14613204

Data Quality

Data completeness: 81%