Agarose Gel Analysis of 15–40-kb PCR Amplimers

Valerie Burland; Foner P. Curtis; Noriko Kusukawa

doi:10.2144/96211pf01

Abstract

Accurate resolution of PCR products in the range of 15-40 kb may be obtained in agarose gels without pulsed field electrophoresis. A gel of 0.3% SeaKem Gold agarose cast on GelBond support film provides good resolution and sufficient get strength to reliably allow staining and photography. This paper describes a test system for Long PCR and demonstrates analysis of the PCR products on a gel run under standard low-voltage electrophoresis conditions.

Keywords

AgaroseAgarose gel electrophoresisGel electrophoresisColor markerChromatographyElectrophoresisPulsed-field gel electrophoresisMolecular biologyResolution (logic)StainingPolyacrylamide gel electrophoresisBiologyChemistryDNAGel electrophoresis of proteinsBiochemistryComputer scienceGeneticsEnzymeGene

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Publication Info

Year: 1996
Type: article
Volume: 21
Issue: 1
Pages: 142-144
Citations: 4
Access: Closed

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APA Style

                            
                                    Valerie Burland, 
                                
                                    Foner P. Curtis, 
                                
                                    Noriko Kusukawa
                                
                            (1996). 
                            Agarose Gel Analysis of 15–40-kb PCR Amplimers. 
                            BioTechniques
                            , 21
                            (1)
                            , 142-144.
                            https://doi.org/10.2144/96211pf01

Identifiers

DOI: 10.2144/96211pf01