Abstract
Gradient-purified resting B lymphocytes can be polyclonally stimulated by antigen-specific major histocompatibility complex (MHC)-restricted helper T lymphocytes as well as by antigen-activated helper T-cell supernatant. In contrast to what has been described so far, we show that helper T-cell supernatant (in the absence of any other added stimulus, such as that provided by anti-mu antibodies) is sufficient to induce both proliferation of resting B cells and their differentiation into IgM-secreting cells. The stimulation induced by the helper T-cell supernatant takes place in serum-free medium and is not MHC-restricted. Our findings strongly support the existence of a B-cell activating factor acting on the resting B cell and causing it to enter the G1 phase of the cell cycle in a MHC-unrestricted manner.
Keywords
BiologyMajor histocompatibility complexPolyclonal antibodiesAntigenT cellclone (Java method)B cellT helper cellMolecular biologyNaive B cellCytotoxic T cellImmunologyCellCellular differentiationAntigen-presenting cellCell biologyAntibodyImmune systemIn vitroBiochemistry
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Publication Info
- Year
- 1984
- Type
- article
- Volume
- 81
- Issue
- 20
- Pages
- 6491-6495
- Citations
- 48
- Access
- Closed
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Cite This
Lise Leclercq,
Georges Bismuth,
Jacques Thèze
(1984).
Antigen-specific helper T-cell clone supernatant is sufficient to induce both polyclonal proliferation and differentiation of small resting B lymphocytes..
Proceedings of the National Academy of Sciences
, 81
(20)
, 6491-6495.
https://doi.org/10.1073/pnas.81.20.6491
Identifiers
- DOI
- 10.1073/pnas.81.20.6491