Abstract
A matrix-assisted laser desorption/ionization (MALDI) ion trap mass spectrometer of new design is described. The instrument is based on a commercial Finnegan LCQ ion trap mass spectrometer to which we have added a MALDI ion source that incorporates a sample stage constructed from a compact disk and a new ion transmission interface. The ion interface contains a quadrupole ion guide installed between the skimmer and the octapoles of the original instrument configuration, allowing for operation in both MALDI and electrospray ionization modes. The instrument has femtomole sensitivity for peptides and is capable of collecting a large number of MALDI MS and MALDI MS/MS spectra within a short period of time. The MALDI source produces reproducible signals for 10(4)-10(5) laser pulses, enabling us to collect MS/MS spectra from all the discernible singly charged ions detected in a MS peptide map. We describe the different modes of the instrument operation and algorithms for data processing as applied to challenging protein identification problems.
Keywords
ChemistryMass spectrometryIon trapHybrid mass spectrometerMALDI imagingQuadrupole ion trapIon sourceIonQuadrupole mass analyzerMatrix-assisted laser desorption/ionizationAmbient ionizationIonizationAnalytical Chemistry (journal)ReflectronQuadrupoleTriple quadrupole mass spectrometerSpectrometerMass spectrumTop-down proteomicsIon trappingSelected reaction monitoringTandem mass spectrometryTime-of-flight mass spectrometryChromatographyDesorptionChemical ionizationAtomic physicsOpticsPhysics
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Publication Info
- Year
- 2001
- Type
- article
- Volume
- 73
- Issue
- 21
- Pages
- 5066-5077
- Citations
- 144
- Access
- Closed
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Cite This
Andrew N. Krutchinsky,
Markus Kalkum,
Brian T. Chait
(2001).
Automatic Identification of Proteins with a MALDI-Quadrupole Ion Trap Mass Spectrometer.
Analytical Chemistry
, 73
(21)
, 5066-5077.
https://doi.org/10.1021/ac010682o
Identifiers
- DOI
- 10.1021/ac010682o