Abstract

Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants.

Keywords

Caenorhabditis elegansBiologyGenomeGeneticsGenetic screenMutantDNA sequencingWhole genome sequencingMutationForward geneticsGeneComputational biology

MeSH Terms

AllelesAnimalsCaenorhabditis elegansDNAHelminthGenomeHelminthMutationPolymorphismSingle Nucleotide

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Publication Info

Year
2008
Type
article
Volume
5
Issue
10
Pages
865-867
Citations
240
Access
Closed

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Cite This

Sumeet Sarin, Snehit Prabhu, M O’Meara et al. (2008). Caenorhabditis elegans mutant allele identification by whole-genome sequencing. Nature Methods , 5 (10) , 865-867. https://doi.org/10.1038/nmeth.1249

Identifiers

DOI
10.1038/nmeth.1249
PMID
18677319
PMCID
PMC2574580

Data Quality

Data completeness: 86%