Abstract
Abstract C57BL/10ScN (nu/nu) mice have B cells and macrophages unresponsive to a phenol-water extracted preparation of Escherichia coli K235 LPS. This unresponsiveness was demonstrated in vitro by the inability of spleen cells to incorporate 3H-thymidine after a 48 hr incubation with LPS (Ph) and by the inability of LPS (Ph) to inhibit macrophage phagocytosis of 51Cr-labeled, opsonized sheep erythrocytes. Furthermore, macrophage cultures stimulated with LPS (Ph) produced low levels of LAF and PGE2 when compared with macrophages from the LPS-sensitive C3H/HeN and C3H/HeN (nu/nu) strains. Therefore, the C57BL/10ScN (nu/nu) strain is similar in its LPS unresponsiveness to the well-characterized C3H/HeJ and C57BL/10ScCR strains. The combination of endotoxin unresponsiveness and the athymic nature of this mouse strain may provide a powerful new tool for studying the cellular events mediating endotoxicity.
Keywords
Strain (injury)PhagocytosisSpleenMacrophageIn vitroIncubationEscherichia coliMolecular biologyOpsoninMicrobiologyLipopolysaccharideThymidineChemistryBiologyImmunologyBiochemistryGene
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Publication Info
- Year
- 1979
- Type
- article
- Volume
- 122
- Issue
- 2
- Pages
- 619-622
- Citations
- 88
- Access
- Closed
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Cite This
Stefanie N. Vogel,
Carl T. Hansen,
David L. Rosenstreich
(1979).
Characterization of a Congenitally LPS-Resistant, Athymic Mouse Strain.
The Journal of Immunology
, 122
(2)
, 619-622.
https://doi.org/10.4049/jimmunol.122.2.619
Identifiers
- DOI
- 10.4049/jimmunol.122.2.619