Abstract
Clinical metagenomic next-generation sequencing (mNGS), the comprehensive analysis of microbial and host genetic material (DNA and RNA) in samples from patients, is rapidly moving from research to clinical laboratories. This emerging approach is changing how physicians diagnose and treat infectious disease, with applications spanning a wide range of areas, including antimicrobial resistance, the microbiome, human host gene expression (transcriptomics) and oncology. Here, we focus on the challenges of implementing mNGS in the clinical laboratory and address potential solutions for maximizing its impact on patient care and public health.
Keywords
MetagenomicsMicrobiomeDNA sequencingComputational biologyInfectious disease (medical specialty)Clinical microbiologyMedicineBioinformaticsDiseaseBiologyGeneInternal medicineGeneticsMicrobiology
MeSH Terms
AnimalsAnti-Bacterial AgentsBacteriaCommunicable DiseasesDNADrug ResistanceMultipleBacterialFungiHelminthsHigh-Throughput Nucleotide SequencingHost-Pathogen InteractionsHumansMedical Laboratory ScienceMetagenomeMetagenomicsPublic HealthViruses
Affiliated Institutions
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Publication Info
- Year
- 2019
- Type
- review
- Volume
- 20
- Issue
- 6
- Pages
- 341-355
- Citations
- 1422
- Access
- Closed
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Cite This
Charles Y. Chiu,
Steven A. Miller
(2019).
Clinical metagenomics.
Nature Reviews Genetics
, 20
(6)
, 341-355.
https://doi.org/10.1038/s41576-019-0113-7
Identifiers
- DOI
- 10.1038/s41576-019-0113-7
- PMID
- 30918369
- PMCID
- PMC6858796