Abstract
A novel orange fluorescent protein, with excitation and emission maxima at 548 and 565 nm, respectively, from the Cnidaria tube anemone Cerianthus sp. has been cloned and overexpressed in Escherichia coli. The orange fluorescent protein has been crystallized by the sitting-drop vapour-diffusion method at 290 K using polyethylene glycol 3350 as a precipitant. A complete set of diffraction data was collected to 2.0 A resolution at 100 K. The crystals belong to the space group R3, with hexagonal unit-cell parameters a = b = 216.947, c = 51.839 A. There are four protein molecules in the asymmetric unit, giving a Matthews coefficient of 2.3 A(3) Da(-1) and a solvent content of 47%.
Keywords
FluorescenceOrange (colour)SolventCrystallographyPolyethylene glycolCrystallizationChemistryBiochemistryOpticsPhysicsOrganic chemistry
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Publication Info
- Year
- 2004
- Type
- article
- Volume
- 60
- Issue
- 2
- Pages
- 340-341
- Citations
- 13
- Access
- Closed
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Cite This
Denis Tsz-Ming Ip,
Siu‐Hong Chan,
Mark D. Allen
et al.
(2004).
Crystallization and preliminary crystallographic analysis of a novel orange fluorescent protein from the<i>Cnidaria</i>tube anemone<i>Cerianthus</i>sp..
Acta Crystallographica Section D Biological Crystallography
, 60
(2)
, 340-341.
https://doi.org/10.1107/s0907444903027100
Identifiers
- DOI
- 10.1107/s0907444903027100