Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing

Keywords

BiologyAlternative splicingRNA splicingExonspliceTranscriptomeComputational biologyGene expression profilingRNA-SeqGeneticsGeneComplementary DNAHuman genomeGene expressionGenomeRNA

Affiliated Institutions

University of Toronto CA

Related Publications

A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome

Marc Sultan , Marcel H. Schulz , Hugues Richard +13 more

The functional complexity of the human transcriptome is not yet fully elucidated. We report a high-throughput sequence of the human transcriptome from a human embryonic kidney a...

2008 Science 1294 citations

Noisy Splicing Drives mRNA Isoform Diversity in Human Cells

Joseph K. Pickrell , Athma A. Pai , Yoav Gilad +1 more

While the majority of multiexonic human genes show some evidence of alternative splicing, it is unclear what fraction of observed splice forms is functionally relevant. In this ...

2010 PLoS Genetics 318 citations

A quantitative atlas of polyadenylation in five mammals

Adnan Derti , Philip W. Garrett-Engele , Kenzie D. MacIsaac +6 more

We developed PolyA-seq, a strand-specific and quantitative method for high-throughput sequencing of 3′ ends of polyadenylated transcripts, and used it to globally map polyadenyl...

2012 Genome Research 649 citations

miRGator v3.0: a microRNA portal for deep sequencing, expression profiling and mRNA targeting

Sooyoung Cho , Insu Jang , Yukyung Jun +10 more

Biogenesis and molecular function are two key subjects in the field of microRNA (miRNA) research. Deep sequencing has become the principal technique in cataloging of miRNA reper...

2012 Nucleic Acids Research 165 citations

<i>Oases:</i>robust<i>de novo</i>RNA-seq assembly across the dynamic range of expression levels

Marcel H. Schulz , Daniel R. Zerbino , Martin Vingron +1 more

Abstract Motivation: High-throughput sequencing has made the analysis of new model organisms more affordable. Although assembling a new genome can still be costly and difficult,...

2012 Bioinformatics 1452 citations

Publication Info

Year: 2008
Type: article
Volume: 40
Issue: 12
Pages: 1413-1415
Citations: 3859
Access: Closed

External Links

View on DOI.org

Social Impact

Altmetric

Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing

PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

3859

OpenAlex

Cite This

APA Style

                            
                                
                                    Qun Pan, 
                                
                                    Ofer Shai, 
                                
                                    Leo J. Lee
                                
                                et al.
                            
                            (2008). 
                            Deep surveying of alternative splicing complexity in the human transcriptome by high-throughput sequencing. 
                            Nature Genetics
                            , 40
                            (12)
                            , 1413-1415.
                            https://doi.org/10.1038/ng.259
                        

Identifiers

DOI: 10.1038/ng.259