Detection of PCR products using self-probing amplicons and fluorescence

Keywords

AmpliconFluorophoreFluorescencePrimer extensionPrimer (cosmetics)Quenching (fluorescence)Förster resonance energy transferMultiplex ligation-dependent probe amplificationMolecular diagnosticsChemistryBiological systemNanotechnologyBase sequencePolymerase chain reactionMaterials scienceBiologyBioinformaticsBiochemistryDNAPhysics

Affiliated Institutions

Related Publications

Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic Sequences

Stephen J. Scharf , Glenn T. Horn , M Kellis

A method is described for directly cloning enzymatically amplified segments of genomic DNA into an M13 vector for sequence analysis. A 110-base pair fragment of the human β-glob...

1986 Science 757 citations

Ligation-independent cloning of PCR products (LIC-PCR)

Charalampos Aslanidis , Pieter J. de Jong

A new procedure has been developed for the efficient cloning of complex PCR mixtures, resulting in libraries exclusively consisting of recombinant clones. Recombinants are gener...

1990 Nucleic Acids Research 1192 citations

Single-Molecule Fluorescence Analysis in Solution

Richard A. Keller , W. Patrick Ambrose , Peter M. Goodwin +3 more

Over the past five years, several groups have developed the capability to detect and identify single fluorescent molecules in solution as the molecules flow through a focused la...

1996 Applied Spectroscopy 207 citations

Publication Info

Year: 1999
Type: article
Volume: 17
Issue: 8
Pages: 804-807
Citations: 695
Access: Closed

External Links

View on DOI.org

Social Impact

Altmetric

Detection of PCR products using self-probing amplicons and fluorescence

PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

695

OpenAlex

Cite This

APA Style

                            
                                
                                    David Whitcombe, 
                                
                                    Jane Theaker, 
                                
                                    Simon P. Guy
                                
                                et al.
                            
                            (1999). 
                            Detection of PCR products using self-probing amplicons and fluorescence. 
                            Nature Biotechnology
                            , 17
                            (8)
                            , 804-807.
                            https://doi.org/10.1038/11751
                        

Identifiers

DOI: 10.1038/11751