Abstract
We describe the synthesis of a family of arylamine-substituted papers which can be diazotized and coupled with nucleic acids. The synthesis is simple and uses readily obtainable starting materials. A partial characterization of the nucleic acid binding activity is reported, as well as a demonstration of the utility of the activated paper for the detection of electrophoretically separated RNA by blot transfer and hybridization.
Keywords
Nucleic acidBiologyNucleic acid thermodynamicsRNABiochemistryNucleic acid structureCombinatorial chemistryMolecular biologyChemistryGene
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Publication Info
- Year
- 1982
- Type
- article
- Volume
- 10
- Issue
- 5
- Pages
- 1799-1810
- Citations
- 218
- Access
- Closed
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Cite This
Brian Seed
(1982).
Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids.
Nucleic Acids Research
, 10
(5)
, 1799-1810.
https://doi.org/10.1093/nar/10.5.1799
Identifiers
- DOI
- 10.1093/nar/10.5.1799