Figure 2 from Human Papillomavirus 42 Drives Digital Papillary Adenocarcinoma and Elicits a Germ Cell–like Program Conserved in HPV-Positive Cancers

Abstract

<p>HPV42 induces transformation <i>in vitro</i> and tumor growth <i>in vivo</i>. <b>A,</b> Growth curves of HPKs transduced with the full genome of HPV11 (low-risk), HPV16 (high-risk), and HPV42. <b>B,</b> Crystal violet staining of MEF cells at day 14 after transduction. Scale bars, 100 μm. <b>C,</b> HPV42-E7 protein interaction partners identified in BioID proximity-labeling experiment. Pathway members of gene ontology terms G<sub>1</sub>–S transition (red) and HIPPO pathway (blue) are labeled. FC, fold change. <b>D,</b> E2F reporter assay in U2OS cells transduced with E7 proteins of HPV11, 16, or 42. ***, <i>P</i> < 0.001; ****, <i>P</i> < 0.0001 (one-way ANOVA, Tukey multiple comparisons test). <b>E,</b> Gene set enrichment analysis (GSEA) of the Molecular Signatures Database (MSigDB) hallmark gene set (top) and MSigDB curated gene set (bottom) comparing HPV42-E7 versus EV-transduced HPK cells. dev., development; NES, normalized enrichment score. <b>F,</b> IHC for YAP1 in a DPA tumor. Scale bar, main: 50 μm; inset: 3 mm. <b>G,</b> Individual tumor growth of EV- or E7 of HPV11-, HPV16-, or HPV42-transduced HaCaT cells in NSG mice. Each group consists of 5 mice (i.e., 10 tumors).</p>

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