Figure 3 from Human Colon Cancer–Derived <i>Clostridioides difficile</i> Strains Drive Colonic Tumorigenesis in Mice

Abstract

<p><i>C. difficile</i> induces the activation of myeloid cells and IL17-producing lymphoid subsets. Multiparameter flow cytometry analysis of colonic lamina propria innate (myeloid and ILCs) and adaptive immune cell populations of LPL isolated from colons collected at 2 weeks p.i. from GF <i>Apc<sup>Min/+</sup></i> mice gavaged with the 3728T isolates with (<i>n</i> = 5) or without (<i>n</i> = 6) <i>C. difficile</i> strain CIm_3728T. Results from two independent experiments. <b>A,</b> Opt-SNE projection of myeloid and ILC populations. <b>B,</b> Dot plots of myeloid cell type abundance as a percentage of the CD45 population. <b>C,</b> Representative gating of DCs, PMN, and IL17 expression by ILC3 cells. <b>D,</b> Opt-SNE projection of adaptive populations (B and T lymphocytes). <b>E,</b> Dot plots of changes in abundance of lymphoid populations with <i>C. difficile</i>. <b>F,</b> Representative gating of lymphoid subsets. Opt-SNE projections are used for visualization purposes only. Statistical analyses in <b>B</b> and <b>E</b> represent Mann–Whitney <i>t</i> tests based on the gating in <b>C</b> and <b>F</b>, respectively. DC, dendritic cells; EILP, early innate lymphoid progenitors; ILC, innate lymphoid cells; IMC, innate myeloid cells; LPL, lamina propria leukocytes; MAC, macrophages; MO, monocytic cells; NK, natural killer; PMN, polymorphonuclear cells; Treg, regulatory T cells.</p>

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