Abstract
The genome size of Vibrio cholerae has been determined by pulsed field gel electrophoresis following digestion of chromosomal DNA with endonucleases. The genome size of all the classical strains examined was about 3000 kb and that of El Tor biotype was 2500 kb. The NotI and SfiI digestion patterns of the genomes of several V. cholerae strains belonging to different serovars and biotypes showed distinct restriction fragment length polymorphism (RFLP). RFLP analysis together with the genome size can be used to differentiate strains of different serovars and biotypes of V. cholerae.
Keywords
Restriction fragment length polymorphismBiologyGenomeRestriction enzymeTerminal restriction fragment length polymorphismGenome sizeVibrio choleraeGeneticsSerotypeRestriction fragmentRestriction mapPolymorphism (computer science)Gel electrophoresisDNAGenePlasmidMicrobiologyPolymerase chain reactionGenotypeBacteria
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Publication Info
- Year
- 1994
- Type
- article
- Volume
- 115
- Issue
- 2-3
- Pages
- 329-334
- Citations
- 28
- Access
- Closed
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Cite This
Susanta Roy Choudhury,
Rupak K. Bhadra,
Jyotirmoy Das
(1994).
Genome size and restriction fragment length polymorphism analysis of<i>Vibrio cholerae</i>strains belonging to different serovars and biotypes.
FEMS Microbiology Letters
, 115
(2-3)
, 329-334.
https://doi.org/10.1111/j.1574-6968.1994.tb06659.x
Identifiers
- DOI
- 10.1111/j.1574-6968.1994.tb06659.x