Genomic instability and endoreduplication triggered by RAD17 deletion

Abstract

Cell cycle checkpoints are critical for genomic stability. Rad17, a component of the checkpoint clamp loader complex (Rad17/Rfc2-5), is required for the response to DNA damage and replication stress. To explore the role of Rad17 in the maintenance of genomic integrity, we established somatic conditional alleles of RAD17 in human cells. We find that RAD17 is not only important for the Atr-mediated checkpoint but is also essential for cell viability. Cells lacking RAD17 exhibited acute chromosomal aberrations and underwent endoreduplication at a high rate. Therefore, RAD17 links the checkpoint to ploidy control and is essential for the maintenance of chromosomal stability.

Keywords

BiologyEndoreduplicationG2-M DNA damage checkpointGenome instabilityCell cycle checkpointDNA damageGeneticsCell biologyDNA repairCHEK1Somatic cellCell cycleDNAGene

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Publication Info

Year: 2003
Type: article
Volume: 17
Issue: 8
Pages: 965-970
Citations: 70
Access: Closed

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APA Style

                            
                                
                                    Xin Wang, 
                                
                                    Lee Zou, 
                                
                                    Huyong Zheng
                                
                                et al.
                            
                            (2003). 
                            Genomic instability and endoreduplication triggered by <i>RAD17</i> deletion. 
                            Genes & Development
                            , 17
                            (8)
                            , 965-970.
                            https://doi.org/10.1101/gad.1065103
                        

Identifiers

DOI: 10.1101/gad.1065103