Keywords
Homologous recombinationIn vitro recombinationRecombinant DNADNARecombinationBiologyExonucleaseInsert (composites)Cloning (programming)FLP-FRT recombinationLigationMolecular cloningGeneticsComputational biologyMolecular biologyGenetic recombinationComputer scienceMaterials scienceGenePolymeraseComplementary DNA
Affiliated Institutions
Related Publications
Ligation-independent cloning of PCR products (LIC-PCR)
A new procedure has been developed for the efficient cloning of complex PCR mixtures, resulting in libraries exclusively consisting of recombinant clones. Recombinants are gener...
Transient and Stable Expression of the Firefly Luciferase Gene in Plant Cells and Transgenic Plants
The luciferase gene from the firefly, Photinus pyralis , was used as a reporter of gene expression by light production in transfected plant cells and transgenic plants. A comple...
Cell-free cloning using φ29 DNA polymerase
We describe conditions for rolling-circle amplification (RCA) of individual DNA molecules 5–7 kb in size by >10 9 -fold, using φ29 DNA polymerase. The principal difficulty wi...
Publication Info
- Year
- 2007
- Type
- article
- Volume
- 4
- Issue
- 3
- Pages
- 251-256
- Citations
- 960
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
960
OpenAlex
Cite This
Mamie Z. Li,
Stephen J. Elledge
(2007).
Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC.
Nature Methods
, 4
(3)
, 251-256.
https://doi.org/10.1038/nmeth1010
Identifiers
- DOI
- 10.1038/nmeth1010