Abstract
Our objective was to clarify the DNA-binding activity of transcription factor NF-kappa B as related to cytokine induction in the synovium of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Synovial membranes were obtained during arthroplasty of the knee from 7 patients with RA and 4 patients with OA. Nuclear extracts obtained from the synovial membranes were examined by electrophoretic mobility shift assay to determine the DNA-binding activity of NF-kappa B. Markedly high DNA-binding activity of NF-kappa B was detected in the synovial membranes of RA patients, while virtually no activity was observed in those of OA patients. These results suggest that the high induction of NF-kappa B in the nuclear extracts may be relatively specific to synovial membranes in RA. NF-kappa B may regulate the production of cytokines at the site of synovial inflammation.
Keywords
Rheumatoid arthritisTranscription factorSynovial membraneOsteoarthritisSynovial fluidNFKB1KappaNF-κBMedicineElectrophoretic mobility shift assayArthritisDNAInflammationImmunologyCytokineMolecular biologyPathologyInternal medicineChemistryGeneBiochemistryBiology
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Publication Info
- Year
- 1995
- Type
- article
- Volume
- 37
- Issue
- 5
- Pages
- 827-32
- Citations
- 75
- Access
- Closed
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Cite This
Hiroshi Asahara,
Masato Asanuma,
N Ogawa
et al.
(1995).
High DNA-binding activity of transcription factor NF-kappa B in synovial membranes of patients with rheumatoid arthritis..
PubMed
, 37
(5)
, 827-32.