Abstract
DNA site-specific recombinases (SSRs) such as Cre, FLPe, and phiC31, are powerful tools for analyzing gene function in vertebrates. While the availability of multiple high-efficiency SSRs would facilitate a wide array of genomic engineering possibilities, efficient recombination in mammalian cells has only been observed with Cre recombinase. Here we report the de novo synthesis of mouse codon-optimized FLP (FLPo) and PhiC31 (PhiC31o) SSRs, which result in recombination efficiencies similar to Cre.
Keywords
RecombinaseSite-specific recombinationBiologyRecombinationCre recombinaseComputational biologyHomologous recombinationGeneticsCre-Lox recombinationFLP-FRT recombinationDNAGeneTransgeneGenetic recombination
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Publication Info
- Year
- 2007
- Type
- article
- Volume
- 2
- Issue
- 1
- Pages
- e162-e162
- Citations
- 392
- Access
- Closed
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Cite This
Christopher S. Raymond,
Philippe Soriano
(2007).
High-Efficiency FLP and ΦC31 Site-Specific Recombination in Mammalian Cells.
PLoS ONE
, 2
(1)
, e162-e162.
https://doi.org/10.1371/journal.pone.0000162
Identifiers
- DOI
- 10.1371/journal.pone.0000162