Human Haploid Cell Genetics Reveals Roles for Lipid Metabolism Genes in Nonapoptotic Cell Death

Abstract

Little is known about the regulation of nonapoptotic cell death. Using massive insertional mutagenesis of haploid KBM7 cells we identified nine genes involved in small-molecule-induced nonapoptotic cell death, including mediators of fatty acid metabolism (ACSL4) and lipid remodeling (LPCAT3) in ferroptosis. One novel compound, CIL56, triggered cell death dependent upon the rate-limiting de novo lipid synthetic enzyme ACC1. These results provide insight into the genetic regulation of cell death and highlight the central role of lipid metabolism in nonapoptotic cell death.

Keywords

Programmed cell deathBiologyGeneLipid metabolismInsertional mutagenesisCell biologyCellMutagenesisCell fate determinationPloidyGeneticsMutationApoptosisBiochemistryGenomeTranscription factor

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Publication Info

Year: 2015
Type: article
Volume: 10
Issue: 7
Pages: 1604-1609
Citations: 964
Access: Closed

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APA Style

                            
                                
                                    Scott J. Dixon, 
                                
                                    Georg E. Winter, 
                                
                                    Leila Musavi
                                
                                et al.
                            
                            (2015). 
                            Human Haploid Cell Genetics Reveals Roles for Lipid Metabolism Genes in Nonapoptotic Cell Death. 
                            ACS Chemical Biology
                            , 10
                            (7)
                            , 1604-1609.
                            https://doi.org/10.1021/acschembio.5b00245
                        

Identifiers

DOI: 10.1021/acschembio.5b00245